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Anti-AP1M2 Polyclonal Antibody

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Catalog No. TMAB-02718

Anti-AP1M2 Polyclonal Antibody is a Rabbit antibody targeting AP1M2. Anti-AP1M2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-AP1M2 Polyclonal Antibody

Anti-AP1M2 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02718
Anti-AP1M2 Polyclonal Antibody is a Rabbit antibody targeting AP1M2. Anti-AP1M2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-AP1M2 Polyclonal Antibody is a Rabbit antibody targeting AP1M2. Anti-AP1M2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Pig,Cow,Horse,Sheep)
Verified Activity
1. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-AP1M2 Polyclonal Antibody, Unconjugated (TMAB-02718) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti-AP1M2 (TMAB-02718) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human AP1M2
Antigen Species
Human
Gene Name
AP1M2
Gene ID
Protein Name
AP-1 complex subunit mu-2
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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