Anti-ANP32B Antibody (3M372) is a Mouse antibody targeting ANP32B. Anti-ANP32B Antibody (3M372) can be used in WB,ICC/IF.

IgG2a, Kappa

3M372

Human

1. Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-02601
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Lysate: HeLa, MCF-7, JurKat
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 29 kDa
Observed MW: 29 kDa

WB: 1:500-1000; ICC/IF: 1:100-500

Monoclonal

ANP32B

Acidic leucine-rich nuclear phosphoprotein 32 family member B

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.