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Anti-Anoctamin-1/ANO1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00124
Alias Tumor amplified and overexpressed sequence 2, Transmembrane protein 16A(eight membrane spanning domains), Transmembrane protein 16A, TMEM16A, TMEM 16A, TAOS2, TAOS 2, ORAOV2, ORAOV 2, Oral cancer overexpressed protein 2, Oral cancer overexpressed 2, Membrane protein, FLJ10261, DOG1, DOG 1, Discovered on gastrointestinal stromal tumors protein 1, Anoctamin1, Anoctamin 1 calcium activated chloride channel, Anoctamin 1, ANO1
Anti-Anoctamin-1/ANO1 Polyclonal Antibody is a Rabbit antibody targeting Anoctamin-1/ANO1. Anti-Anoctamin-1/ANO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Anoctamin-1/ANO1 Polyclonal Antibody
Anti-Anoctamin-1/ANO1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00124Alias Tumor amplified and overexpressed sequence 2, Transmembrane protein 16A(eight membrane spanning domains), Transmembrane protein 16A, TMEM16A, TMEM 16A, TAOS2, TAOS 2, ORAOV2, ORAOV 2, Oral cancer overexpressed protein 2, Oral cancer overexpressed 2, Membrane protein, FLJ10261, DOG1, DOG 1, Discovered on gastrointestinal stromal tumors protein 1, Anoctamin1, Anoctamin 1 calcium activated chloride channel, Anoctamin 1, ANO1
Anti-Anoctamin-1/ANO1 Polyclonal Antibody is a Rabbit antibody targeting Anoctamin-1/ANO1. Anti-Anoctamin-1/ANO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Anoctamin-1/ANO1 Polyclonal Antibody is a Rabbit antibody targeting Anoctamin-1/ANO1. Anti-Anoctamin-1/ANO1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Tumor amplified and overexpressed sequence 2, Transmembrane protein 16A(eight membrane spanning domains), Transmembrane protein 16A, TMEM16A, TMEM 16A, TAOS2, TAOS 2, ORAOV2, ORAOV 2, Oral cancer overexpressed protein 2, Oral cancer overexpressed 2, Membrane protein, FLJ10261, DOG1, DOG 1, Discovered on gastrointestinal stromal tumors protein 1, Anoctamin1, Anoctamin 1 calcium activated chloride channel, Anoctamin 1, ANO1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated (TMAB-00124) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated (TMAB-00124) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Skeletal muscle (Mouse) Lysate at 40 μg Lane 2: Skeletal muscle (Rat) Lysate at 40 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: A673 (Human) Cell Lysate at 30 μg Lane 5: U-2OS (Human) Cell Lysate at 30 μg Lane 6: MKN45 (Human) Cell Lysate at 30 μg Primary: Anti-ANO1/TMEM16A (TMAB-00124) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114-120 kDa Observed band size: 120 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | TMEM16A (Transmembrane protein 16A) is an eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products and is amplified in various cancers. It acts as a calcium-activated chloride channel and is required for normal tracheal development. |
| Immunogen | KLH conjugated synthetic peptide: human DOG-1/TMEM16A |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Tumor Associated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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