Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

1. Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated (TMAB-00124) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated (TMAB-00124) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Skeletal muscle (Mouse) Lysate at 40 μg
Lane 2: Skeletal muscle (Rat) Lysate at 40 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A673 (Human) Cell Lysate at 30 μg
Lane 5: U-2OS (Human) Cell Lysate at 30 μg
Lane 6: MKN45 (Human) Cell Lysate at 30 μg
Primary: Anti-ANO1/TMEM16A (TMAB-00124) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114-120 kDa
Observed band size: 120 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DOG-1/TMEM16A

Human

55107

Tumor Associated