Anti-Annexin V Polyclonal Antibody is a Rabbit antibody targeting Annexin V. Anti-Annexin V Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (TMAB-02597) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (TMAB-02597) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (TMAB-02597) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
4. Tissue/cell: human Neurological glioblastoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (TMAB-02597) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
5. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated (TMAB-02597) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
6. Sample: Lung (Mouse) Lysate at 40 μg
Primary: Anti-Annexin V (TMAB-02597) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
7. Blank control: RSC96 (blue).
Primary Antibody: Rabbit Anti-Annexin V antibody (TMAB-02597), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (TMAB-02597, 1 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of TMAB-02597 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
8. Sample:
PANC-1 (Human) Cell Lysate at 30 μg
Primary: Anti-Annexin V (TMAB-02597) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD
9. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lung (Mouse) Lysate at 40 μg
Primary: Anti-Annexin V (TMAB-02597) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD
10. Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Mouse Uterus tissue lysates
Lane 4: Mouse Spleen tissue lysates
Lane 5: Mouse Stomach tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Rat Placenta tissue lysates
Lane 8: Rat Spleen tissue lysates
Lane 9: Human HepG2 cell lysates
Lane 10: Human Panc-1 cell lysates
Primary:
Anti-Annexin V (TMAB-02597) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

recombind human Annexin V

Human

ANXA5

Annexin A5

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.