Anti-Annexin A7 Antibody (1E442) is a Rabbit antibody targeting Annexin A7. Anti-Annexin A7 Antibody (1E442) can be used in IF, IHC-Fr, IHC-P, IP, WB.

IgG

1E442

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with Annexin A7 monoclonal antibody, unconjugated (TMAB-02592) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Annexin A7 Monoclonal Antibody, Unconjugated (TMAB-02592) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Annexin A7 Monoclonal Antibody, Unconjugated (TMAB-02592) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Annexin A7 Monoclonal Antibody, Unconjugated (TMAB-02592) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Annexin A7 Monoclonal Antibody, Unconjugated (TMAB-02592) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human Annexin A7

Human

ANXA7

Annexin A7

Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.