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Anti-Annexin A3 Polyclonal Antibody

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Catalog No. TMAB-02589

Anti-Annexin A3 Polyclonal Antibody is a Rabbit antibody targeting Annexin A3. Anti-Annexin A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Annexin A3 Polyclonal Antibody

Anti-Annexin A3 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02589
Anti-Annexin A3 Polyclonal Antibody is a Rabbit antibody targeting Annexin A3. Anti-Annexin A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Annexin A3 Polyclonal Antibody is a Rabbit antibody targeting Annexin A3. Anti-Annexin A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-Annexin A3 (TMAB-02589) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin III Polyclonal Antibody, Unconjugated (TMAB-02589) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundVertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Annexin III
Antigen Species
Human
Gene Name
ANXA3
Gene ID
Protein Name
Annexin A3
Uniprot ID
Function
Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
Chemical Properties
Molecular WeightTheoretical: 36 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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