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Anti-Annexin A13 Polyclonal Antibody

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Catalog No. TMAB-02586

Anti-Annexin A13 Polyclonal Antibody is a Rabbit antibody targeting Annexin A13. Anti-Annexin A13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Annexin A13 Polyclonal Antibody

Anti-Annexin A13 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02586
Anti-Annexin A13 Polyclonal Antibody is a Rabbit antibody targeting Annexin A13. Anti-Annexin A13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Annexin A13 Polyclonal Antibody is a Rabbit antibody targeting Annexin A13. Anti-Annexin A13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Horse,Rabbit)
Verified Activity
1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Annexin A13 Polyclonal Antibody, Unconjugated (TMAB-02586) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-Annexin A13 (TMAB-02586) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane. Associated with the plasma membrane of undifferentiated, proliferating crypt epithelial cells as well as differentiated villus enterocytes.
Tissue SpecificityGut specific.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe Annexins constitute a family of structurally-related, relatively abundant proteins that exhibit Ca2+-dependent binding to phospholipids. Annexins function in multiple aspects of cell biology including regulation of membrane trafficking, transmembrane channel activity, inhibition of phospholipase A2, inhibition of coagulation and mediation of cell-matrix interactions. Annexin A13 is considered the original progenitor of the 12 members of vertebrate Annexins. The expression of Annexin A13 is highly tissue-specific, being expressed only in intestinal and kidney epithelial cells. This expression is associated with a highly differentiated intracellular transport function. Two alternative splicing isoforms of Annexin A13 exist, both of which bind to rafts.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Annexin A13
Antigen Species
Human
Gene Name
ANXA13
Gene ID
Protein Name
Annexin A13
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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