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Anti-Anillin Polyclonal Antibody
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Catalog No. TMAB-02566
Anti-Anillin Polyclonal Antibody is a Rabbit antibody targeting Anillin. Anti-Anillin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Anillin Polyclonal Antibody
Anti-Anillin Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-02566
Anti-Anillin Polyclonal Antibody is a Rabbit antibody targeting Anillin. Anti-Anillin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Anillin Polyclonal Antibody is a Rabbit antibody targeting Anillin. Anti-Anillin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (Rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (TMAB-02566) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 10. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human U251 cell lysates Lane 4: Human MCF-7 cell lysates Primary: Anti-Anillin (TMAB-02566) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 125 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. |
| Tissue Specificity | Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. |
| Immunogen | KLH conjugated synthetic peptide: human Anillin |
| Antigen Species | Human |
| Gene Name | ANLN |
| Gene ID | |
| Protein Name | Anillin |
| Uniprot ID | |
| Function | Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. |
| Molecular Weight | Theoretical: 124 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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