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Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody
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Catalog No. TMAB-00120
Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody is a Rabbit antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody
Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00120
Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody is a Rabbit antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human (predicted:Mouse,Rat,Chicken,Dog,Pig) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Pig) |
| Verified Activity | 1. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Angiopoietin 2 (TMAB-00120) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa 2. Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Ang2 Polyclonal Antibody, Unconjugated (TMAB-00120) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Angiopoietin 2 |
| Antigen Species | Human |
| Gene ID | 285 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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