Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody is a Rabbit antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig)

1. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-Angiopoietin 2 (TMAB-00120) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
2. Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Ang2 Polyclonal Antibody, Unconjugated (TMAB-00120) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Angiopoietin 2

Human

ANGPT2

Angiopoietin-2

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.