Antibody Type: Rabbit Monoclonal

Application: WB, ICC/IF, IP

Reactivity: Human

Rabbit IgG

7Q134

Human

1. Immunofluorescence staining of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ang2 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
2. Anti-Ang2 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: A549 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:57 kDa.
-Observed band size:70 kDa.
3. Ang2 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-Lane B:0.5 mg A549 Whole Cell Lysate.
-Lane C:0.5 mg 293T Whole Cell Lysate.
-0.5 µL anti-Ang2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-Ang2 rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 57 kDa.
-Observed band size: 57 kDa

WB,ICC/IF,IP

WB: 1:500-1:1000; ICC-IF: 1:100-1:500; IP: 0.2-1 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human Angiopoietin-2 / ANG 2 protein

Human

Cancer Drug Targets