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Anti-AMPK α1 Antibody (7H670)
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Catalog No. TMAC-00178
Alias wu:fa94c10, Tau protein kinase PRKAA1, SNF1-like protein AMPK, SNF1A, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, OTTHUMP00000161796, OTTHUMP00000161795, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, Hydroxymethylglutaryl CoA reductase kinase, hormone sensitive lipase kinase, HMGCR kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKalpha1, AMPKa1, AMPK1, AMPK subunit alpha-1, AMPK alpha1, AMPK alpha 1 chain, AMPK alpha 1, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha -1, AMP -activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, ACACA kinase, AAPK1_HUMAN, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit
Anti-AMPK α1 Antibody (7H670) is a Rabbit antibody targeting AMPK α1. Anti-AMPK α1 Antibody (7H670) can be used in WB,ICC/IF,IP,FCM.
Anti-AMPK α1 Antibody (7H670)
Anti-AMPK α1 Antibody (7H670)
😃Good
Catalog No. TMAC-00178Alias wu:fa94c10, Tau protein kinase PRKAA1, SNF1-like protein AMPK, SNF1A, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, OTTHUMP00000161796, OTTHUMP00000161795, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, Hydroxymethylglutaryl CoA reductase kinase, hormone sensitive lipase kinase, HMGCR kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKalpha1, AMPKa1, AMPK1, AMPK subunit alpha-1, AMPK alpha1, AMPK alpha 1 chain, AMPK alpha 1, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha -1, AMP -activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, ACACA kinase, AAPK1_HUMAN, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit
Anti-AMPK α1 Antibody (7H670) is a Rabbit antibody targeting AMPK α1. Anti-AMPK α1 Antibody (7H670) can be used in WB,ICC/IF,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-AMPK α1 Antibody (7H670) is a Rabbit antibody targeting AMPK α1. Anti-AMPK α1 Antibody (7H670) can be used in WB,ICC/IF,IP,FCM. |
| Synonyms | wu:fa94c10, Tau protein kinase PRKAA1, SNF1-like protein AMPK, SNF1A, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, OTTHUMP00000161796, OTTHUMP00000161795, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, Hydroxymethylglutaryl CoA reductase kinase, hormone sensitive lipase kinase, HMGCR kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKalpha1, AMPKa1, AMPK1, AMPK subunit alpha-1, AMPK alpha1, AMPK alpha 1 chain, AMPK alpha 1, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha -1, AMP -activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, ACACA kinase, AAPK1_HUMAN, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of AMPK alpha 1 on different lysates using anti-AMPK alpha 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: MCF-7. 2. ICC staining AMPK alpha 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining AMPK alpha 1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining AMPK alpha 1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with AMPK alpha 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 63 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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