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Anti-Amphiphysin Polyclonal Antibody

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Catalog No. TMAB-02523 Copy Product Info
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Anti-Amphiphysin Polyclonal Antibody is a Rabbit antibody targeting Amphiphysin. Anti-Amphiphysin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Amphiphysin Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2207-10 days7-10 days
100 µL$3727-10 days7-10 days
200 µL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Amphiphysin Polyclonal Antibody is a Rabbit antibody targeting Amphiphysin. Anti-Amphiphysin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Chicken,Dog,Cow,Horse,Rabbit)
Verified Activity
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Amphiphysin Polyclonal Antibody, Unconjugated (TMAB-02523) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.
Tissue SpecificityNeurons, certain endocrine cell types and spermatocytes.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundAmphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Amphiphysin
Antigen Species
Human
Gene Name
AMPH
Gene ID
Protein Name
Amphiphysin
Uniprot ID
Function
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Chemical Properties
Molecular WeightTheoretical: 76 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-Amphiphysin Polyclonal Antibody molecular weight