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Anti-Aminopeptidase N/CD13 Antibody (8T409)

😃Good
Catalog No. TMAY-00182
Alias P150, Cd13, AP-N, Apn, AP-M, alanyl (membrane) aminopeptidase

Anti-Aminopeptidase N/CD13 Antibody (8T409) is a Mouse antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (8T409) can be used in WB,IP.

Anti-Aminopeptidase N/CD13 Antibody (8T409)

Anti-Aminopeptidase N/CD13 Antibody (8T409)

😃Good
Catalog No. TMAY-00182Alias P150, Cd13, AP-N, Apn, AP-M, alanyl (membrane) aminopeptidase
Anti-Aminopeptidase N/CD13 Antibody (8T409) is a Mouse antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (8T409) can be used in WB,IP.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Aminopeptidase N/CD13 Antibody (8T409) is a Mouse antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (8T409) can be used in WB,IP.
AliasP150, Cd13, AP-N, Apn, AP-M, alanyl (membrane) aminopeptidase
Ig Type
Monoclonal Mouse IgG1
Clone
8T409
Reactivity
Human
Verified Activity
1. Anti-ANPEP mouse monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell Lysate.

-Lane B: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:110 kDa.

-Observed band size:140 kDa.

2. ANPEP-His was immunoprecipitated using:

-Lane A:0.5 mg HEPG2 Whole Cell Lysate.

-4 μL anti-ANPEP-His mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

-Primary antibody:

-Anti-ANPEP-His mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size: 150 kDa.

-Observed band size:150 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 5-10 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD13 /ANPEP (rh CD13 /ANPEP; TMPY-00665; NP_001141.2; Lys69-Lys967,603Met/Ile). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundAminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human CD13 /ANPEP Protein (TMPY-00665)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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