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Anti-AMACR Antibody (3H209)

Catalog No. TMAB-02505 Copy Product Info
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Anti-AMACR Antibody (3H209) is a Rabbit antibody targeting AMACR. Anti-AMACR Antibody (3H209) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-AMACR Antibody (3H209)

Copy Product Info
🥰Excellent
Catalog No. TMAB-02505

Anti-AMACR Antibody (3H209) is a Rabbit antibody targeting AMACR. Anti-AMACR Antibody (3H209) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-AMACR Antibody
(3H209)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1497-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-AMACR Antibody (3H209) is a Rabbit antibody targeting AMACR. Anti-AMACR Antibody (3H209) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
3H209
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated (TMAB-02505) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated (TMAB-02505) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated (TMAB-02505) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated (TMAB-02505) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated (TMAB-02505) at 1:100 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with AMACR monoclonal antibody, unconjugated (TMAB-02505) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationPeroxisome. Mitochondrion.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
Antigen Details
Gene Name
AMACR
Gene ID
Protein Name
alpha-methylacyl-CoA racemase
Function
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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