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Anti-ALOX15 Polyclonal Antibody 2
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Catalog No. TMAB-00099
Anti-ALOX15 Polyclonal Antibody 2 is a Rabbit antibody targeting ALOX15. Anti-ALOX15 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ALOX15 Polyclonal Antibody 2
Anti-ALOX15 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00099
Anti-ALOX15 Polyclonal Antibody 2 is a Rabbit antibody targeting ALOX15. Anti-ALOX15 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: A549 (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-ALOX15 (TMAB-00099) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 77 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:10-50; IF: 1:10-50 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes and synthesis of signaling molecules, as well as inducing structural and metabolic changes in the cell. The Lox enzymes in mammals include 12-LO and 15-LO, which are classified with respect to their positional specificity of the deoxygenation of their most common substrate, arachidonic acid. The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, as well as survival and apoptosis. 15-Lipoxygenase (15-LO) acts in physiological membrane remodeling and the pathogenesis of atherosclerosis, inflammation, and carcinogenesis. It is highly regulated and expressed in a tissue- and cell-type-specific fashion. IL-4 and IL-13 play important roles in transactivating the 15-LO gene. Overexpression of 15-LO type 1 in prostate cancer contributes to the cancer progression by regulating IGF-1R expression and activation. |
| Immunogen | KLH conjugated synthetic peptide: human ALOX15 |
| Antigen Species | Human |
| Gene ID | 246 |
| Uniprot ID | |
| Biology Area | Tumor biomarkers,Lipoprotein metabolism,Metabolism,Fatty acids,Lipoprotein metabolism,Fatty acid oxidation,Heart disease,Hormones |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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