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Anti-ALKBH5 Polyclonal Antibody
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Catalog No. TMAB-00096
Alias RNA demethylase ALKBH5, OFOXD1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, Alkylated DNA repair protein alkB homolog 5, ALKBH5, ABH5
Anti-ALKBH5 Polyclonal Antibody is a Rabbit antibody targeting ALKBH5. Anti-ALKBH5 Polyclonal Antibody can be used in WB.
Anti-ALKBH5 Polyclonal Antibody
Anti-ALKBH5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00096Alias RNA demethylase ALKBH5, OFOXD1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, Alkylated DNA repair protein alkB homolog 5, ALKBH5, ABH5
Anti-ALKBH5 Polyclonal Antibody is a Rabbit antibody targeting ALKBH5. Anti-ALKBH5 Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ALKBH5 Polyclonal Antibody is a Rabbit antibody targeting ALKBH5. Anti-ALKBH5 Polyclonal Antibody can be used in WB. |
| Synonyms | RNA demethylase ALKBH5, OFOXD1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, Alkylated DNA repair protein alkB homolog 5, ALKBH5, ABH5 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Rabbit) |
| Verified Activity | Sample: Testis (Rat) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Primary: Anti-ALKBH5 (TMAB-00096) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35-44 kDa Observed band size: 40 kDa  |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus speckle |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export and is required for spermatogenesis. |
| Immunogen | KLH conjugated synthetic peptide: human ALKBH5 |
| Antigen Species | Human |
| Gene Name | ALKBH5 |
| Gene ID | |
| Protein Name | RNA demethylase ALKBH5 |
| Uniprot ID | |
| Biology Area | DNA Damage Response |
| Function | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A)RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity). |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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