Anti-ALKBH1 Antibody (3E211) is a Rabbit antibody targeting ALKBH1. Anti-ALKBH1 Antibody (3E211) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

3E211

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with ALKBH1 monoclonal antibody, unconjugated (TMAB-02447) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, Unconjugated (TMAB-02447) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; IF: 1:100-200

Monoclonal

A synthesized peptide: human ALKBH1

Human

ALKBH1

Nucleic acid dioxygenase ALKBH1