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Anti-Alkaline Phosphatase/ALPL Antibody (9A742) is a Rabbit antibody targeting Alkaline Phosphatase/ALPL. Anti-Alkaline Phosphatase/ALPL Antibody (9A742) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $269 | 7-10 days |
Description | Anti-Alkaline Phosphatase/ALPL Antibody (9A742) is a Rabbit antibody targeting Alkaline Phosphatase/ALPL. Anti-Alkaline Phosphatase/ALPL Antibody (9A742) can be used in WB. |
Alias | TNSALP, TNAP, HOPS, AP-TNAP, APTNAP, alkaline phosphatase, liver/bone/kidney |
Ig Type | Monoclonal Rabbit IgG |
Clone | 9A742 |
Reactivity | Human |
Verified Activity | Anti-Alkaline Phosphatase/ALPL rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:57 kDa. -Observed band size:100 kDa |
Application | WB |
Recommended Dose | WB: 1:500-1:2000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL |
Antigen Species | Human |
Biology Area | Phosphatases and Regulators |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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