Anti-ALDOA Antibody (4A872) is a Rabbit antibody targeting ALDOA. Anti-ALDOA Antibody (4A872) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG/Kappa

4A872

Human,Mouse,Rat

1. Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-02441
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF7, 2: HepG2, 3: Mouse kidney, 4: Rat brain
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 39 kDa
Observed MW: 35 kDa
3. Tissue: Human colon cancer
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02441

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

ALDOA

Fructose-bisphosphate aldolase A

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.