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Anti-ALDOA Antibody (4A872)

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Catalog No. TMAB-02441

Anti-ALDOA Antibody (4A872) is a Rabbit antibody targeting ALDOA. Anti-ALDOA Antibody (4A872) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-ALDOA Antibody (4A872)

Anti-ALDOA Antibody (4A872)

Copy Product Info
😃Good
Catalog No. TMAB-02441
Anti-ALDOA Antibody (4A872) is a Rabbit antibody targeting ALDOA. Anti-ALDOA Antibody (4A872) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ALDOA Antibody (4A872) is a Rabbit antibody targeting ALDOA. Anti-ALDOA Antibody (4A872) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG/Kappa
Clone
4A872
Reactivity
Human,Mouse,Rat
Verified Activity
1. Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-02441
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF7, 2: HepG2, 3: Mouse kidney, 4: Rat brain
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 39 kDa
Observed MW: 35 kDa
3. Tissue: Human colon cancer
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02441
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundResearch areas:Cancer //Cancer Metabolism //Metabolic signaling pathway < Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein. Cellular localization:Cytoplasmic Tissue Specificity:adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue.
Antigen Details
Gene Name
ALDOA
Gene ID
Protein Name
Fructose-bisphosphate aldolase A
Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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