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Anti-ALDH1L1 Antibody (8I450) is a Rabbit antibody targeting ALDH1L1. Anti-ALDH1L1 Antibody (8I450) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
| Description | Anti-ALDH1L1 Antibody (8I450) is a Rabbit antibody targeting ALDH1L1. Anti-ALDH1L1 Antibody (8I450) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG/Kappa |
| Clone | 8I450 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-02436 2. 25 μg total protein per lane of various lysates (see on figure) probed with ALDH1L1 monoclonal antibody, unconjugated (TMAB-02436) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Highly expressed in liver, pancreas and kidney. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] |
| Immunogen | A synthesized peptide: human ALDH1L1 |
| Gene Name | ALDH1L1 |
| Gene ID | |
| Protein Name | Cytosolic 10-formyltetrahydrofolate dehydrogenase |
| Molecular Weight | Theoretical: 100 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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