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Anti-ALDH1L1 Antibody (8I450)

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Catalog No. TMAB-02436

Anti-ALDH1L1 Antibody (8I450) is a Rabbit antibody targeting ALDH1L1. Anti-ALDH1L1 Antibody (8I450) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-ALDH1L1 Antibody (8I450)

Anti-ALDH1L1 Antibody (8I450)

Copy Product Info
😃Good
Catalog No. TMAB-02436
Anti-ALDH1L1 Antibody (8I450) is a Rabbit antibody targeting ALDH1L1. Anti-ALDH1L1 Antibody (8I450) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ALDH1L1 Antibody (8I450) is a Rabbit antibody targeting ALDH1L1. Anti-ALDH1L1 Antibody (8I450) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG/Kappa
Clone
8I450
Reactivity
Human,Mouse,Rat
Verified Activity
1. Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-02436
2. 25 μg total protein per lane of various lysates (see on figure) probed with ALDH1L1 monoclonal antibody, unconjugated (TMAB-02436) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ALDH1L1 Monoclonal Antibody, Unconjugated (TMAB-02436) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityHighly expressed in liver, pancreas and kidney.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
Antigen Details
Immunogen
A synthesized peptide: human ALDH1L1
Gene Name
ALDH1L1
Gene ID
Protein Name
Cytosolic 10-formyltetrahydrofolate dehydrogenase
Chemical Properties
Molecular WeightTheoretical: 100 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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