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Anti-ALDH1A1 Antibody (5U880)
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Catalog No. TMAB-02432
Anti-ALDH1A1 Antibody (5U880) is a Rabbit antibody targeting ALDH1A1. Anti-ALDH1A1 Antibody (5U880) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ALDH1A1 Antibody (5U880)
Anti-ALDH1A1 Antibody (5U880)
Copy Product Info😃Good
Catalog No. TMAB-02432
Anti-ALDH1A1 Antibody (5U880) is a Rabbit antibody targeting ALDH1A1. Anti-ALDH1A1 Antibody (5U880) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ALDH1A1 Antibody (5U880) is a Rabbit antibody targeting ALDH1A1. Anti-ALDH1A1 Antibody (5U880) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 5U880 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Mouse Lung tissue lysates Lane 4: Mouse Kidney tissue lysates Primary: Anti-ALDH1A1 (TMAB-02432) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 46 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (TMAB-02432) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (TMAB-02432) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (TMAB-02432) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (TMAB-02432) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (TMAB-02432) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Expressed in the liver, lung, and testis. Apparently not expressed at detectable levels in kidney, stomach, ovary, heart, and brain. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]. |
| Immunogen | KLH conjugated synthetic peptide: human ALDH1A1 |
| Antigen Species | Human |
| Gene Name | ALDH1A1 |
| Gene ID | |
| Protein Name | Retinal dehydrogenase 1 |
| Uniprot ID | |
| Function | Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. |
| Molecular Weight | Theoretical: 55 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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