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Anti-ALAS1 Antibody (7J767)
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Catalog No. TMAC-00130
Alias nonspecific, mitochondrial, EC 2.3.1.37, Delta-aminolevulinate synthase 1, Delta-ALA synthase 1, ALAS-H, ALAS3 ALASH, ALAS 1, 5-aminolevulinic acid synthase 1, 5-aminolevulinate synthase
Anti-ALAS1 Antibody (7J767) is a Rabbit antibody targeting ALAS1. Anti-ALAS1 Antibody (7J767) can be used in ICC/IF,WB.
Anti-ALAS1 Antibody (7J767)
Anti-ALAS1 Antibody (7J767)
😃Good
Catalog No. TMAC-00130Alias nonspecific, mitochondrial, EC 2.3.1.37, Delta-aminolevulinate synthase 1, Delta-ALA synthase 1, ALAS-H, ALAS3 ALASH, ALAS 1, 5-aminolevulinic acid synthase 1, 5-aminolevulinate synthase
Anti-ALAS1 Antibody (7J767) is a Rabbit antibody targeting ALAS1. Anti-ALAS1 Antibody (7J767) can be used in ICC/IF,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-ALAS1 Antibody (7J767) is a Rabbit antibody targeting ALAS1. Anti-ALAS1 Antibody (7J767) can be used in ICC/IF,WB. |
| Synonyms | nonspecific, mitochondrial, EC 2.3.1.37, Delta-aminolevulinate synthase 1, Delta-ALA synthase 1, ALAS-H, ALAS3 ALASH, ALAS 1, 5-aminolevulinic acid synthase 1, 5-aminolevulinate synthase |
| Ig Type | IgG |
| Clone | 7J767 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR, Lane 2: HUVEC, Lane 3: Hela. 2. ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 71 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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