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Anti-Alas1 Antibody (2R254)
Catalog No. TMAB-02425 Copy Product Info
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Anti-Alas1 Antibody (2R254) is a Rabbit antibody targeting Alas1. Anti-Alas1 Antibody (2R254) can be used in WB.
Anti-Alas1 Antibody (2R254)
Copy Product Info🥰Excellent
Catalog No. TMAB-02425
Anti-Alas1 Antibody (2R254) is a Rabbit antibody targeting Alas1. Anti-Alas1 Antibody (2R254) can be used in WB.
Anti-Alas1 Antibody
(2R254)
(2R254)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Alas1 Antibody (2R254) is a Rabbit antibody targeting Alas1. Anti-Alas1 Antibody (2R254) can be used in WB. |
| Ig Type | IgG |
| Clone | 2R254 |
| Reactivity | Human |
| Verified Activity | Sample: Lane 1: JAR (Human) Cell Lysate at 30 μg Lane 2: Huvec (Human) Cell Lysate at 30 μg Lane 3: Hela (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Lane 5: MCF-7 (Human) Cell Lysate at 30 μg Lane 6: K562 (Human) Cell Lysate at 30 μg Lane 7: Raji (Human) Cell Lysate at 30 μg Primary: Anti-Alas1 (TMAB-02425) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 71/65 kD Observed band size: 65 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion matrix |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. |
| Immunogen | Recombinant Protein: human Alas1 C-terminal 200aa |
| Antigen Species | Human |
| Gene Name | ALAS1 |
| Gene ID | |
| Protein Name | 5-aminolevulinate synthase, nonspecific, mitochondrial |
| Uniprot ID |
| Molecular Weight | Theoretical: 65 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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