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Anti-ALAS-E Polyclonal Antibody
Catalog No. TMAB-02427 Copy Product Info
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Anti-ALAS-E Polyclonal Antibody is a Rabbit antibody targeting ALAS-E. Anti-ALAS-E Polyclonal Antibody can be used in WB.
Anti-ALAS-E Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-02427
Anti-ALAS-E Polyclonal Antibody is a Rabbit antibody targeting ALAS-E. Anti-ALAS-E Polyclonal Antibody can be used in WB.
Anti-ALAS-E Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-ALAS-E Polyclonal Antibody is a Rabbit antibody targeting ALAS-E. Anti-ALAS-E Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Sample: Lane 1: Human MOLT4 cell lysates Lane 2: Human K562 cell lysates Primary: Anti-ALAS-E (TMAB-02427) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kDa Observed band size: 62 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion matrix |
| Tissue Specificity | Erythroid specific. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. |
| Immunogen | KLH conjugated synthetic peptide: human ALAS2/ALAS-E |
| Antigen Species | Human |
| Gene Name | ALAS2 |
| Gene ID | |
| Protein Name | 5-aminolevulinate synthase, erythroid-specific, mitochondrial |
| Uniprot ID |
| Molecular Weight | Theoretical: 59 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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