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Anti-AKT3 Polyclonal Antibody is a Rabbit antibody targeting AKT3. Anti-AKT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-AKT3 Polyclonal Antibody is a Rabbit antibody targeting AKT3. Anti-AKT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKT3) Polyclonal Antibody, Unconjugated (TMAB-02423) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Testis (Mouse) Lysate at 40 μg Primary: Anti-AKT3 (TMAB-02423) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 60 kD 3. Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human SH-SY5Y cell lysates Primary: Anti-AKT3 (TMAB-02423) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 60 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Immunogen | KLH conjugated synthetic peptide: human AKT3 |
| Antigen Species | Human |
| Gene Name | AKT3 |
| Protein Name | RAC-gamma serine/threonine-protein kinase |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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