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Anti-AKT3 Polyclonal Antibody

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Catalog No. TMAB-02423

Anti-AKT3 Polyclonal Antibody is a Rabbit antibody targeting AKT3. Anti-AKT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-AKT3 Polyclonal Antibody

Anti-AKT3 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02423
Anti-AKT3 Polyclonal Antibody is a Rabbit antibody targeting AKT3. Anti-AKT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-AKT3 Polyclonal Antibody is a Rabbit antibody targeting AKT3. Anti-AKT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKT3) Polyclonal Antibody, Unconjugated (TMAB-02423) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Testis (Mouse) Lysate at 40 μg
Primary: Anti-AKT3 (TMAB-02423) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 60 kD
3. Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Primary: Anti-AKT3 (TMAB-02423) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human AKT3
Antigen Species
Human
Gene Name
AKT3
Protein Name
RAC-gamma serine/threonine-protein kinase
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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