Anti-AKR1C1/2 Antibody (8B279) is a Rabbit antibody targeting AKR1C1/2. Anti-AKR1C1/2 Antibody (8B279) can be used in WB,FCM.

IgG

8B279

Human,Mouse,Rat

1. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-AKR1C1/2 antibody (TMAB-02407, 1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with AKR1C1/2 monoclonal antibody, unconjugated (TMAB-02407) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1:50-100

Monoclonal

A synthesized peptide: human AKR1C1

Human

AKR1C1

Aldo-keto reductase family 1 member C1

Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.