Anti-AKAP7 Polyclonal Antibody is a Rabbit antibody targeting AKAP7. Anti-AKAP7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Pig,Horse,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKAP7) Polyclonal Antibody, Unconjugated (TMAB-02402) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-AKAP7 Polyclonal Antibody, Unconjugated (TMAB-02402) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human AKAP7

Human

AKAP7

A-kinase anchor protein 7 isoforms alpha and beta

Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel.