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Anti-AKAP14 Antibody (9H736) is a Rabbit antibody targeting AKAP14. Anti-AKAP14 Antibody (9H736) can be used in ICC/IF,IP,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-AKAP14 Antibody (9H736) is a Rabbit antibody targeting AKAP14. Anti-AKAP14 Antibody (9H736) can be used in ICC/IF,IP,WB. |
Synonyms | Protein kinase A anchoring protein 14, PRKA14, A-kinase anchoring protein 28, AKAP28, AKAP 14, A kinase anchor protein 28 kDa, A kinase anchor protein 14, A kinase (PRKA) anchor protein 14 |
Ig Type | IgG |
Clone | 9H736 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: A549. 2. ICC staining AKAP14 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining AKAP14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining AKAP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Binds to type II regulatory subunits of protein kinase A and anchors/targets them. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 23 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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