Anti-AIMP2 Polyclonal Antibody is a Rabbit antibody targeting AIMP2. Anti-AIMP2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Rat (predicted:Mouse,Pig,Cow,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIMP2) Polyclonal Antibody, Unconjugated (TMAB-02396) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (AIMP2) polyclonal Antibody, Unconjugated (TMAB-02396) 1: 25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human AIMP2

Human

AIMP2

Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Required for assembly and stability of the minoacyl-tRNA synthase complex. Mediates ubiquitination and egradation of FUBP1, a transcriptional activator of MYC, leading o MYC down-regulation which is required for aveolar type II cell ifferentiation. Blocks MDM2-mediated ubiquitination and egradation of p53/TP53. Functions as a proapoptotic factor.