Anti-AGPAT4 Polyclonal Antibody is a Rabbit antibody targeting AGPAT4. Anti-AGPAT4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-AGPAT4 Polyclonal Antibody, Unconjugated (TMAB-02384) 1: 200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Brain (Rat) Lysate at 40 μg
Heart (Rat) Lysate at 40 μg
Primary: Anti-AGPAT4 (TMAB-02384) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 44 kD
Observed band size: 41 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Agpat4

Human

AGPAT4

1-acyl-sn-glycerol-3-phosphate acyltransferase delta

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity).