Anti-AFAP1L2 Polyclonal Antibody is a Rabbit antibody targeting AFAP1L2. Anti-AFAP1L2 Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse (predicted:Human,Rat)

1. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-AFAP1L2 (TMAB-02373) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90 kD
2. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-AFAP1L2 antibody (TMAB-02373)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human AFAP1L2

Human

AFAP1L2

Actin filament-associated protein 1-like 2

May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation.