Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat (predicted:Horse,Rabbit)

IgG

Human,Mouse,Rat (predicted:Horse,Rabbit)

1. Sample:
Lane 1: Mouse Urinary bladder tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Stomach tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human U-2 OS cell lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human SH-SY5Y cell lysates
Primary: Anti-ADRB3 (TMAB-00073) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa
2. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (TMAB-00073) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (TMAB-00073) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
mesentery (mouse) Lysate at 40 μg
lympHnode (Mouse) Lysate at 40 μg
Primary: Anti-ADRB3/beta 3 Adrenergic Receptor (TMAB-00073) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ADRB3

Human

155