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Anti-ADRA2C Polyclonal Antibody

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Catalog No. TMAB-02357

Anti-ADRA2C Polyclonal Antibody is a Rabbit antibody targeting ADRA2C. Anti-ADRA2C Polyclonal Antibody can be used in WB,FCM.

Anti-ADRA2C Polyclonal Antibody

Anti-ADRA2C Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02357
Anti-ADRA2C Polyclonal Antibody is a Rabbit antibody targeting ADRA2C. Anti-ADRA2C Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ADRA2C Polyclonal Antibody is a Rabbit antibody targeting ADRA2C. Anti-ADRA2C Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Pig)
Verified Activity
1. Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ADRA2C antibody (TMAB-02357), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Blank control (blue line): A431 (fixed with 70% ice-cold methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-ADRA2C/PE-CY5 Conjugated antibody (TMAB-02357-PE-CY5), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY5.
3. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human SH-SY5Y cell lysate
Lane 4: Human 293T cell lysates
Lane 5: Human A549 cell lysates
Primary: Anti-ADRA2C (TMAB-02357) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 58 kDa
Application
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundAlpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ADRA2C/alpha 2C Adrenergic Receptor
Antigen Species
Human
Gene Name
ADRA2C
Gene ID
Protein Name
alpha-2C adrenergic receptor
Uniprot ID
Function
The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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