Anti-ADK Antibody (7G880) is a Rabbit antibody targeting ADK. Anti-ADK Antibody (7G880) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

7G880

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: PC-3, 3: Human liver, 4: Mouse liver, 5: Rat liver
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 41 kDa
Observed MW: 41 kDa
2. Tissue: Human tonsil
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02346
3. Tissue: Human liver
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02346
4. Tissue: Rat liver
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02346
5. Tissue: Mouse liver
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02346

WB: 1:500-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

KLH conjugated synthetic peptide: human ADK

Human

ADK

Adenosine kinase

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.