Anti-ADIPOR2 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR2. Anti-ADIPOR2 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow)

1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (TMAB-00065) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (TMAB-00065) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-Adiponectin receptor 2 antibody (TMAB-00065, Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (TMAB-00065, 0.2 μg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature.
4. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Skeletal muscle (Mouse) Lysate at 40 μg
Lane 3: Heart (Rat) Lysate at 40 μg
Lane 4: Skeletal muscle (Rat) Lysate at 40 μg
Lane 5: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-Adiponectin receptor 2 (TMAB-00065) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 43 kDa
5. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (TMAB-00065) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human Adiponectin receptor 2

Human

ADIPOR2

Adiponectin receptor protein 2

Lipid metabolism,Energy Metabolism,Hormones,Heart disease,Diabetes,Cancer,Fatty acid oxidation,Lipoprotein metabolism,Fatty acids,Energy Metabolism,Metabolism,Lipoprotein metabolism,Diabetes associated

Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.