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Anti-ADGRL4 Polyclonal Antibody

Catalog No. TMAB-02333 Copy Product Info
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Anti-ADGRL4 Polyclonal Antibody is a Rabbit antibody targeting ADGRL4. Anti-ADGRL4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-ADGRL4 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-02333

Anti-ADGRL4 Polyclonal Antibody is a Rabbit antibody targeting ADGRL4. Anti-ADGRL4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-ADGRL4 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ADGRL4 Polyclonal Antibody is a Rabbit antibody targeting ADGRL4. Anti-ADGRL4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Horse)
Verified Activity
1. Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ETL Polyclonal Antibody, Unconjugated (TMAB-02333) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
2. Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ETL Polyclonal Antibody, Unconjugated (TMAB-02333) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ETL Polyclonal Antibody, Unconjugated (TMAB-02333) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
Tissue SpecificityMainly expressed in smooth muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe epidermal growth factor (EGF)-TM7 family constitutes a group of leukocyte-restricted, class B G-protein coupled receptors (GPCRs). These include CD97, EMR1(EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL. These family members are characterized by an extended extracellular region with several N-terminal EGF domains and are predominantly expressed on cells of the immune system. Unlike other GPCRs, neither EMR2 nor EMR3 have mouse orthologs. The molecular twins CD97 and EMR2 only differ by 6 out of 236 amino acids, but this slight difference is enough to alter ligand specificity and confer nonredundant functions. EMR3 may function in myeloid-myeloid interactions during immune and inflammatory responses. ETL is a 738 amino acid protein composed of a large extracellular domain with EGF-like repeats, a seven-transmembrane domain, and a short cytoplasmic tail. ETL mRNA expression is up-regulated in the adult rat and human heart.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ADGRL4
Antigen Species
Human
Gene Name
ADGRL4
Gene ID
Protein Name
Adhesion G protein-coupled receptor L4
Uniprot ID
Function
Could be involved in cardiac development.
Chemical Properties
Molecular WeightTheoretical: 76 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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