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Anti-ADGRE1 Antibody (1T88)
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Catalog No. TMAB-02332
Anti-ADGRE1 Antibody (1T88) is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Antibody (1T88) can be used in FCM,WB.
Anti-ADGRE1 Antibody (1T88)
Anti-ADGRE1 Antibody (1T88)
Copy Product Info😃Good
Catalog No. TMAB-02332
Anti-ADGRE1 Antibody (1T88) is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Antibody (1T88) can be used in FCM,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days | |
| 200 μL | $754 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-ADGRE1 Antibody (1T88) is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Antibody (1T88) can be used in FCM,WB. |
| Ig Type | IgG |
| Clone | 1T88 |
| Reactivity | Human |
| Verified Activity | 1. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (TMAB-02332) Dilution: 1 μg/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. 2. Sample: Lane 1: Human THP-1 cell lysates Primary: Anti-ADGRE1 (TMAB-02332) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kDa Observed band size: 100 kDa |
| Application | |
| Recommended Dose | FCM: 1μg/Test; WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. |
| Tissue Specificity | Wide expression; increased levels in peripheral blood mononuclear cells. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages. |
| Immunogen | Recombinant Protein: human ADGRE1 |
| Antigen Species | Human |
| Gene Name | ADGRE1 |
| Gene ID | |
| Protein Name | Adhesion G protein-coupled receptor E1 |
| Uniprot ID | |
| Function | Could be involved in cell-cell interactions. |
| Molecular Weight | Theoretical: 95 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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