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Anti-Adenylosuccinate Lyase Polyclonal Antibody
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Catalog No. TMAB-02331
Anti-Adenylosuccinate Lyase Polyclonal Antibody is a Rabbit antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Adenylosuccinate Lyase Polyclonal Antibody
Anti-Adenylosuccinate Lyase Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-02331
Anti-Adenylosuccinate Lyase Polyclonal Antibody is a Rabbit antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Adenylosuccinate Lyase Polyclonal Antibody is a Rabbit antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: Lane 1: Stomach (Mouse) Lysate at 40 μg Lane 2: Spleen (Mouse) Lysate at 40 μg Lane 3: Jurkat (Human) Cell Lysate at 30 μg Lane 4: HL60 (Human) Cell Lysate at 30 μg Lane 5: 293T (Human) Cell Lysate at 30 μg Primary: Anti-Adenylosuccinate Lyase (TMAB-02331) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55/48 kD Observed band size: 50 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated (TMAB-02331) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated (TMAB-02331) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated (TMAB-02331) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Adenylosuccinate Lyase |
| Antigen Species | Human |
| Gene Name | ADSL |
| Gene ID | |
| Protein Name | Adenylosuccinate lyase |
| Uniprot ID |
| Molecular Weight | Theoretical: 55 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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