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Anti-Adenylate kinase 2 Antibody (7E520)

🥰Excellent
Catalog No. TMAY-02504

Anti-Adenylate kinase 2 Antibody (7E520) is a Rabbit antibody targeting Adenylate kinase 2. Anti-Adenylate kinase 2 Antibody (7E520) can be used in ICC/IF.

Anti-Adenylate kinase 2 Antibody (7E520)

Anti-Adenylate kinase 2 Antibody (7E520)

🥰Excellent
Catalog No. TMAY-02504
Anti-Adenylate kinase 2 Antibody (7E520) is a Rabbit antibody targeting Adenylate kinase 2. Anti-Adenylate kinase 2 Antibody (7E520) can be used in ICC/IF.
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Product Introduction

Bioactivity
Description
Anti-Adenylate kinase 2 Antibody (7E520) is a Rabbit antibody targeting Adenylate kinase 2. Anti-Adenylate kinase 2 Antibody (7E520) can be used in ICC/IF.
Ig Type
Monoclonal Rabbit IgG
Clone
7E520
Reactivity
Human
Verified Activity
Immunofluorescence staining of AK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human AK2 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Application
ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human AK2 / Adenylate kinase 2 (rh AK2 / Adenylate kinase 2; P54819-1Met1-Ile239).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundAdenylate kinase 2 (AK2) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Adenylate kinase2 (AK2) is expressed in mitochondrial intermembrane space. It may play a role in apoptosis. It has been demonstrated that in apoptotic cells AK2 was translocated into the cytosol concomitantly with cytochronme C. Mutations in this gene are the cause of reticular dysgenesis. These mutations result in absent or strongly decreased protein expression. It has been also established that AK2 is specifically expressed in the stria vascularis region of the inner ear, which provides an explanation of the sensorineural deafness in these individuals.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human AK2 / Adenylate kinase 2 Protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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