Anti-Adenylate cyclase 1 Polyclonal Antibody is a Rabbit antibody targeting Adenylate cyclase 1. Anti-Adenylate cyclase 1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenylate cyclase 1) Polyclonal Antibody, Unconjugated (TMAB-00060) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
MCF-7 Cell (Human) lysate at 30 μg
Primary: Anti-ADCY1 (TMAB-00060) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 123 kDa
3. Sample:
A549 Cell (Human) Lysate at 30 μg
Primary: Anti-ADCY1 (TMAB-00060) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 123 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human adenylate cyclase type I

Human

ADCY1

Adenylate cyclase type 1

Integration of energy metabolism,Integration of energy,Cancer,Regulation,Regulators

This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.