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Anti-Adenylate cyclase 1 Polyclonal Antibody
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Catalog No. TMAB-00060
Anti-Adenylate cyclase 1 Polyclonal Antibody is a Rabbit antibody targeting Adenylate cyclase 1. Anti-Adenylate cyclase 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Adenylate cyclase 1 Polyclonal Antibody
Anti-Adenylate cyclase 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00060
Anti-Adenylate cyclase 1 Polyclonal Antibody is a Rabbit antibody targeting Adenylate cyclase 1. Anti-Adenylate cyclase 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Mouse) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenylate cyclase 1) Polyclonal Antibody, Unconjugated (TMAB-00060) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: MCF-7 Cell (Human) lysate at 30 μg Primary: Anti-ADCY1 (TMAB-00060) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kDa Observed band size: 123 kDa 3. Sample: A549 Cell (Human) Lysate at 30 μg Primary: Anti-ADCY1 (TMAB-00060) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kDa Observed band size: 123 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The membrane bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein coupled receptors. Eight human AC isoforms (AC-1 through AC-4) have been identified up to now and genes for at least nine adenylate cyclase (AC-1-AC-9) have been cloned characterized and sequenced. This is a calmodulin-sensitive adenylyl cyclase which may be involved in regulatory processes in the central nervous system. It may play a role in neuronal plasticity, memory acquisition and learning and is expressed predominantly in the brain, retina and adrenal medulla. |
| Immunogen | KLH conjugated synthetic peptide: human adenylate cyclase type I |
| Antigen Species | Human |
| Gene ID | 107 |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,Integration of energy,Cancer,Regulation,Regulators |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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