Anti-ADAR1 Polyclonal Antibody2 is a Rabbit antibody targeting ADAR1. Anti-ADAR1 Polyclonal Antibody2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ADAR1) Polyclonal Antibody, Unconjugated (TMAB-02309) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
2. Sample:
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-ADAR1 (TMAB-02309) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 110 kD
3. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-ADAR1 (TMAB-02309) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 110 kD
4. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-ADAR1 (TMAB-02309) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 110 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DRADA

Human

ADAR

Double-stranded RNA-specific adenosine deaminase

Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.