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Anti-ADAM17 Polyclonal Antibody
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Catalog No. TMAB-00055
Anti-ADAM17 Polyclonal Antibody is a Rabbit antibody targeting ADAM17. Anti-ADAM17 Polyclonal Antibody can be used in WB,FCM,IHC-P,IHC-Fr,IF,ICC/IF.
Anti-ADAM17 Polyclonal Antibody
Anti-ADAM17 Polyclonal Antibody
😃Good
Catalog No. TMAB-00055
Anti-ADAM17 Polyclonal Antibody is a Rabbit antibody targeting ADAM17. Anti-ADAM17 Polyclonal Antibody can be used in WB,FCM,IHC-P,IHC-Fr,IF,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM,IHC-P,IHC-Fr,IF,ICC/IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (TMAB-00055) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. 25 μg total protein per Lane of various lysates probed with ADAM17 polyclonal antibody, unconjugated (TMAB-00055) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 9. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (ADAM17) polyclonal Antibody, unconjugated (TMAB-00055) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| Application | WB,FCM,IHC-P,IHC-Fr,IF,ICC/IF |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human ADAM17 |
| Antigen Species | Human |
| Gene ID | 6868 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,ADAM protein family,Signal transduction,ADAMs,Hypoxia,Cancer,Neurodegenerative disease,SARS Coronavirus,Alzheimer's disease,Transmembrane,Secreted |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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