Anti-ADAM12 Polyclonal Antibody 2 is a Rabbit antibody targeting ADAM12. Anti-ADAM12 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit)

1. Blank control (Black line): U87MG (Black).
Primary Antibody (green line): Rabbit Anti-ADAM12 antibody (TMAB-02279)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Heart (Mouse) Lysate at 40 μg
Primary: Anti-ADAM12 (TMAB-02279) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77/100 kD
Observed band size: 115 kD

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ADAM12

Human

ADAM12

Disintegrin and metalloproteinase domain-containing protein 12

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).