Anti-ADAM10 Antibody (9D750) is a Mouse antibody targeting ADAM10. Anti-ADAM10 Antibody (9D750) can be used in FCM,IHC-P,IHC-Fr,IF,ICC/IF.

IgG1, Kappa

9D750

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAM10 Monoclonal Antibody, Unconjugated (TMAB-02276) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Right occipital lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAM10 Monoclonal Antibody, Unconjugated (TMAB-02276) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. The Jurkat (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-ADAM10 antibody (TMAB-02276, 1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4. 4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (ADAM10) monoclonal Antibody, unconjugated (TMAB-02276) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM: 1:50-100; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; ICC/IF: 1:50-200

Monoclonal

ADAM10

Disintegrin and metalloproteinase domain-containing protein 10