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Anti-ADAM10 Antibody (7S737)

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Catalog No. TMAB-02277

Anti-ADAM10 Antibody (7S737) is a Rabbit antibody targeting ADAM10. Anti-ADAM10 Antibody (7S737) can be used in WB,IHC-P,IHC-Fr,IF,IP.

Anti-ADAM10 Antibody (7S737)

Anti-ADAM10 Antibody (7S737)

Copy Product Info
😃Good
Catalog No. TMAB-02277
Anti-ADAM10 Antibody (7S737) is a Rabbit antibody targeting ADAM10. Anti-ADAM10 Antibody (7S737) can be used in WB,IHC-P,IHC-Fr,IF,IP.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2617-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ADAM10 Antibody (7S737) is a Rabbit antibody targeting ADAM10. Anti-ADAM10 Antibody (7S737) can be used in WB,IHC-P,IHC-Fr,IF,IP.
Ig Type
IgG
Clone
7S737
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM10 Monoclonal Antibody, Unconjugated (TMAB-02277) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. 25 μg total protein per lane of various lysates (see on figure) probed with ADAM10 monoclonal antibody, unconjugated (TMAB-02277) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; IP: 1:20-50
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand, nuclear after ligand-binding.Isoform Beta: Nucleus. Note=Localized largely in the nucleus.
Tissue SpecificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Concentration1mg/ml
Research BackgroundMembers of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]
Antigen Details
Immunogen
A synthesized peptide: human ADAM 10
Antigen Species
Human
Gene Name
ADAM10
Gene ID
Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 84 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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