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Anti-ADAM10 Antibody (7S737) is a Rabbit antibody targeting ADAM10. Anti-ADAM10 Antibody (7S737) can be used in WB,IHC-P,IHC-Fr,IF,IP.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
| Description | Anti-ADAM10 Antibody (7S737) is a Rabbit antibody targeting ADAM10. Anti-ADAM10 Antibody (7S737) can be used in WB,IHC-P,IHC-Fr,IF,IP. |
| Ig Type | IgG |
| Clone | 7S737 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM10 Monoclonal Antibody, Unconjugated (TMAB-02277) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. 25 μg total protein per lane of various lysates (see on figure) probed with ADAM10 monoclonal antibody, unconjugated (TMAB-02277) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; IP: 1:20-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand, nuclear after ligand-binding.Isoform Beta: Nucleus. Note=Localized largely in the nucleus. |
| Tissue Specificity | Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
| Concentration | 1mg/ml |
| Research Background | Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016] |
| Immunogen | A synthesized peptide: human ADAM 10 |
| Antigen Species | Human |
| Gene Name | ADAM10 |
| Gene ID | |
| Protein Name | Disintegrin and metalloproteinase domain-containing protein 10 |
| Uniprot ID |
| Molecular Weight | Theoretical: 84 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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