Anti-AD7c-NTP Polyclonal Antibody is a Rabbit antibody targeting AD7c-NTP. Anti-AD7c-NTP Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control (black line): U87MG.
Primary Antibody (green line): Rabbit Anti-AD7c-NTP antibody (TMAB-02273)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample: AD7c-NTP recombinant protein (Human) Lysate at 100 ng
Primary: Anti-AD7c-NTP (TMAB-02273) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 46 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Neuronal thread protein AD7c-NTP

Human

AF010144.1

Alzheimer-associated neuronal thread protein (AD7c-NTP)