Anti-ACTR1B Antibody (5N556) is a Rabbit antibody targeting ACTR1B. Anti-ACTR1B Antibody (5N556) can be used in FCM, IP, WB.

IgG

5N556

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with ACTR1B monoclonal antibody, unconjugated (TMAB-02266) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ACTR1B antibody (TMAB-02266, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human ACTR1B

Human

ACTR1B

beta-centractin

Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.