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Anti-ACTH Antibody (2I218)
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Catalog No. TMAB-02256
Anti-ACTH Antibody (2I218) is a Rabbit antibody targeting ACTH. Anti-ACTH Antibody (2I218) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ACTH Antibody (2I218)
Anti-ACTH Antibody (2I218)
Copy Product Info😃Good
Catalog No. TMAB-02256
Anti-ACTH Antibody (2I218) is a Rabbit antibody targeting ACTH. Anti-ACTH Antibody (2I218) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-ACTH Antibody (2I218) is a Rabbit antibody targeting ACTH. Anti-ACTH Antibody (2I218) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 2I218 |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with ACTH monoclonal antibody, unconjugated (TMAB-02256) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated (TMAB-02256) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated (TMAB-02256) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | ACTH and MSH are produced by the pituitary gland. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | PBS, Glycerol, BSA. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. |
| Immunogen | A synthesized peptide: human POMC |
| Antigen Species | Human |
| Gene Name | POMC |
| Gene ID | |
| Protein Name | Pro-opiomelanocortin |
| Uniprot ID | |
| Function | ACTH stimulates the adrenal glands to release cortisol.
MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. |
| Molecular Weight | Theoretical: 4.5/29 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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