Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-ACTA1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00044
Alias Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA

Anti-ACTA1 Polyclonal Antibody is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ACTA1 Polyclonal Antibody

Anti-ACTA1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00044Alias Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA
Anti-ACTA1 Polyclonal Antibody is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5287-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-ACTA1 Polyclonal Antibody is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasAlpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Actin Polyclonal Antibody, Unconjugated (TMAB-00044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Bone (Mouse) Lysate at 40 μg
Primary: Anti-Actin (TMAB-00044) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
3. Sample:
Heart (Mouse) Lysate at 40 μg
Primary: Anti-Actin (TMAB-00044) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
4. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Actin (TMAB-00044) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
5. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Actin Polyclonal Antibody, Unconjugated (TMAB-00044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Actin Polyclonal Antibody, Unconjugated (TMAB-00044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytoskeleton.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ACTA1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Actin,Troponin,Tropomyosin,Actin Crosslinking,Actin Binding Proteins,Actin Assembly,Actin,RIP,RIP
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-ACTA1 Polyclonal Antibody | purchase Anti-ACTA1 Polyclonal Antibody | Anti-ACTA1 Polyclonal Antibody cost | order Anti-ACTA1 Polyclonal Antibody