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Anti-ACTA1 Polyclonal Antibody
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Catalog No. TMAB-00044
Alias Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA
Anti-ACTA1 Polyclonal Antibody is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ACTA1 Polyclonal Antibody
Anti-ACTA1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00044Alias Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA
Anti-ACTA1 Polyclonal Antibody is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ACTA1 Polyclonal Antibody is a Rabbit antibody targeting ACTA1. Anti-ACTA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Alpha-actin-1, alpha skeletal muscle, Actin, alpha skeletal muscle, ACTA1, ACTA |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Actin Polyclonal Antibody, Unconjugated (TMAB-00044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Bone (Mouse) Lysate at 40 μg Primary: Anti-Actin (TMAB-00044) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 3. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-Actin (TMAB-00044) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 4. Sample: Muscle (Mouse) Lysate at 40 μg Primary: Anti-Actin (TMAB-00044) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 5. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Actin Polyclonal Antibody, Unconjugated (TMAB-00044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Actin Polyclonal Antibody, Unconjugated (TMAB-00044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human ACTA1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Actin,Troponin,Tropomyosin,Actin Crosslinking,Actin Binding Proteins,Actin Assembly,Actin,RIP,RIP |
| Molecular Weight | Theoretical: 42 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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