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Anti-ACSS2 Antibody (1N487)
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Catalog No. TMAB-02249
Anti-ACSS2 Antibody (1N487) is a Rabbit antibody targeting ACSS2. Anti-ACSS2 Antibody (1N487) can be used in WB,ICC/IF,FCM.
Anti-ACSS2 Antibody (1N487)
Anti-ACSS2 Antibody (1N487)
Copy Product Info😃Good
Catalog No. TMAB-02249
Anti-ACSS2 Antibody (1N487) is a Rabbit antibody targeting ACSS2. Anti-ACSS2 Antibody (1N487) can be used in WB,ICC/IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-ACSS2 Antibody (1N487) is a Rabbit antibody targeting ACSS2. Anti-ACSS2 Antibody (1N487) can be used in WB,ICC/IF,FCM. |
| Ig Type | IgG |
| Clone | 1N487 |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with ACSS2 monoclonal antibody, unconjugated (TMAB-02249) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ACSS2 antibody (TMAB-02249, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids.Acetate is the preferred substrate.Can also utilize propionate with a much lower affinity,Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis. |
| Immunogen | A synthesized peptide: human ACSS2 |
| Antigen Species | Human |
| Gene Name | ACSS2 |
| Gene ID | |
| Protein Name | Acetyl-coenzyme A synthetase, cytoplasmic |
| Uniprot ID |
| Molecular Weight | Theoretical: 79 kDa. Actual: 79 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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